Chryseobacterium panacis sp. nov., isolated from ginseng soil

Abstract

A novel strain, DCY107T, was isolated from soil collected from a ginseng field in Gochang, Republic of Korea. Strain DCY107T is Gram-negative, yellow pigmented, non-motile, non-flagellate, rod-shaped and aerobic. The strain was found to grow optimally at 25–30 °C and pH 6.5–7. Phylogenetically, strain DCY107T is closely related to Chryseobacterium polytrichastri DSM 26899T (98.49 % 16S rRNA gene sequence similarity), Chryseobacterium yeoncheonense JCM 18516T (97.78 %), Chryseobacterium aahli LMG 27338T (97.74 %), Chryseobacterium limigenitum LMG28734T (97.74 %), Chryseobacterium ginsenosidimutans JCM 16719T (97.47 %) and Chryseobacterium gregarium LMG 24052T (97.31 %). The DNA–DNA relatedness values between strain DCY107T and reference strains were found to be clearly below 70 %. The DNA G+C content of strain DCY107T was determined to be 34.2 mol%. The predominant quinone was identified menaquinone 6 (MK-6). The major polar lipids were identified as phosphatidylethanolamine and unidentified lipids: aminolipids AL1, AL2 and lipid L2. C16:00, iso-C15:00, iso-C15:02OH, iso-C17:03OH and summed feature 9 (iso-C17:1 ω9c and/or C16:0 10-methyl) were identified as the major fatty acids present in strain DCY107T. The results of physiological and biochemical tests allowed strain DCY107T to be differentiated phenotypically from other recognised species belonging to the genus Chryseobacterium. Therefore, it is suggested that the newly isolated organism represents a novel species, for which the name Chryseobacterium panacis sp. nov. is proposed, with the type strain designated as DCY107T (=CCTCC AB 2015195T = KCTC 42750T).