Paenibacillus puernese sp. nov., a β-glucosidase-producing bacterium isolated from Pu’er tea

Abstract

A Gram-staining-positive, endospore-forming, aerobic, rod-shaped bacterium, designated as DCY97T, was isolated from ripened Pu’er tea and was identified by using a polyphasic approach. 16S rRNA gene sequence analysis showed that strain DCY97T was closely related to Paenibacillus dongdonensis KUDC0114T (98.0 %), Paenibacillus oceanisediminis L10T (97.7 %), and Paenibacillus barcinonensis BP-23T (97.2 %). The phenotypic and chemotaxonomic characteristics of strain DCY97T matched with the characteristics of members belonging to the genus Paenibacillus. The major identified polar lipids included phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol. The predominant quinone was MK-7. The major fatty acids were anteiso-C15:0 (35.1 %), anteiso-C16:0 (19.0 %), and iso-C16:0 (13.9 %). The peptidoglycan cell wall was composed of meso-diaminopimelic acids, alanine, and D-glutamic acid. The genomic DNA G + C content was determined to be 46.7 mol%. The DNA–DNA relatedness between strain DCY97T and Paenibacillus dongdonensis KCTC 33221T, Paenibacillus oceanisediminis KACC 16023T, Paenibacillus barcinonensis KCTC 13019T were 27, 19, and 10 %, respectively. Based on the genotypic, phenotypic, and chemotaxonomic characteristics, strain DCY97T is considered as a novel species of the genus Paenibacillus, for which the name Paenibacillus puernese sp. nov. is proposed. The type strain is DCY97T (=KCTC 33596T = JCM 140369T).